Toll-Like Receptors and Cytokines as Surrogate Biomarkers for Evaluating Vaginal Immune Response following Microbicide Administration

Figure 3

RT-PCR analysis of TLR- 3 and 9 expression. Total RNA was extracted from CVE cells and subjected to RT-PCR analysis as described in the Material and Methods. No change in TLR gene expression was observed in nisin administered animals compared to placebo gel-treated group. However, in SDS gel administered animals, the expression of TLR genes was upregulated. House keeping gene, GAPDH expression confirms roughly equivalent loading of RNA samples.