Research Article
Toll-Like Receptors and Cytokines as Surrogate Biomarkers for Evaluating Vaginal Immune Response following Microbicide Administration
Table 1
Oligonucleotide primers used for the study.
| Gene | Accession No | Primer sequence | Product size (bp) | Annealing temperature |
|
IL-4 | DQ_852343 | F-AGTTCTACCTCCACCACAAGGT | 212 |
60 | R-TCAGCTCTGACGCTTTGAGTAT |
|
IL-6 | NM_001082064 | F-AGGAGCTGAGGAAAGAGATGTG | 225 |
58 | R-TGTTTTCTTCGTCACTCCTGAA |
|
IL-10 | NM_001082045 | F-TTCTTTCAATCGAAGGATCAGC | 267 |
59 | R-CTCATTCATGGCTTTGTAGACG |
|
TNF- | ID_AB128153 | F-CTAGCCCACGTAGTAGCAAACC | 159 |
60 | R-GCTGAAGAGAACCTGGGAGTAG |
|
TLR-3 | NM_003265 | F-GAT CTG TCT CAT AAT GGC TTG | 305 |
60 | R-GAC AGA TTC CGA ATG CTT GTG |
|
TLR-9 | NM_017442 | F-TTC CCT GTA GCT GCT GTC C | 207 |
59 | R-ACA GCC AGT TGC AGT TCA CC |
|
Defensin-1 | NM_001082299 | F-CTCTGCTTGCTGCCATTCTC | 196 |
60 | R-AATCGTCTGCAAGTACAGGACAC |
|
GAPDH | NM_01082253 | F-CCATTCATTGACCTCCACTACA | 199 |
59 | R-CGTTGCTGACAATCTTGAGAGA |
|
|