The NALP3/Cryopyrin-Inflammasome Complex is Expressed in LPS-Induced Ocular Inflammation
Figure 1
Histological and molecular analysis
of the LPS-induced ocular inflammation. C3H/HeN mice
were inoculated in the footpad with 200 g/50 L of LPS and the inflammation was analyzed at 3, 6, 12, 24, and 48 hours. Control mice (C) received a footpad injection of 50 L of sterile PBS. (a) histological analysis of leukocyte infiltration in
the anterior chamber stained with hematoxylin-eosin (40x). The arrows point infiltrated cells.
The figure shows a representative result of five mice studied in each time.
(b) average of infiltrated leukocytes observed in (a). (c) average
of semiquantitative analysis of IL-6 and TNFα mRNA expression.
Asterisk indicates significant difference compared with the control ()
analyzed by Kruskal-Wallis and Turkey
tests. (d) ocular expression of
IL1β protein in treated and untreated mice.
Immunostaing of IL-1β of untreated mouse (I) and treated
mice at 12 hours posttreatment (II and III). (e) testis tissue was used as positive control of IL1β
expression; irrelevant antibody (I), IL-1β antibody (II).
Blue staining means positive signal.
