Upregulation of Salmonella-Induced IL-6 Production in Caco-2 Cells by PJ-34, PARP-1 Inhibitor: Involvement of PI3K, p38 MAPK, ERK, JNK, and NF-B
Figure 2
Effect of PJ-34 on Salmonella-induced activation of NF- B. NF-B is a central regulator of the intestinal epithelial cell innate immune response induced by infection with enteroinvasive bacteria. The Western blots illustrate (a) the expression of I-B proteins in cytosolic extracts of Caco-2 cells exposed to wild-type S. Typhimurium strain SL1344 in the absence or presence of PJ-34. Actin works as a normalization of nuclear protein and (b) the expression of p65 NF-kB proteins in nuclear extracts of Caco-2 cells exposed to wild-type S. Typhimurium strain SL1344 in the absence or presence of PJ-34. Actin and LaminA/C work as a normalization of cytosolic and nuclear protein, respectively. The results shown are representative of 3 separate experiments.
