Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy
Figure 5
The levels of P expression (mean fluorescence intensity; MFI values) for (a) T lymphocytes (CD3+P+) and their subpopulations (CD4+P+ and CD8+P+), and (b) NK cells (CD16+P+ and CD56+P+) in peripheral blood from patients subjected to classic or laparoscopic cholecystectomy before (two-colored columns) and after the operation (one-colored column). O-statistical significance between the preoperative values in classic and laparoscopic cholecystectomy; *statistical significance between the preoperative and postoperative values in classic or laparoscopic cholecystectomy. One mark , two marks . (c) Representative flow cytometric profile of perforin expression on the gated CD56+ PBMNLC before and 24 hours after laparoscopic cholecystectomy. The shadow area was perforin expression while the nonshadow area was isotype control. The amount of perforin per cell is indicated by the mean fluorescence intensity (MFI).
