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Mediators of Inflammation
Volume 2009 (2009), Article ID 146872, 8 pages
http://dx.doi.org/10.1155/2009/146872
Research Article

Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation

Department of Rheumatology, University Medical Centre, Vodnikova 62, 1000 Ljubljana, Slovenia

Received 16 July 2009; Accepted 26 October 2009

Academic Editor: Hidde Bult

Copyright © 2009 Katja Lakota et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effects of anti-inflammatory plant extracts, such as black tea extract (BTE) and resveratrol (RSV) could modulate cell activation leading to atherosclerosis, however there is little comparative information about how different endothelial cell types are affected by these compounds. In order to compare human endothelial cells derived from different origins (umbilical vein or HUVEC, coronary artery or HCAEC, microvascular or HMVEC) and their interleukin- (IL- ) responsiveness, IL-6 ELISA, RT-PCR, tissue factor assay, and prostacyclin responses using 6-keto ELISA were determined. The IL- -induced IL-6 levels were dose-dependent with highest responses seen in HCAEC. Significant inhibition of IL- responses was achieved with BTE and RSV, with the largest decrease of IL-6 and TF seen in HCAEC. Prostacyclin levels were highest in HUVEC and were inhibited by RSV in all cell types. The differences between the endothelial cell types could account for greater susceptibility of coronary arteries to inflammation and atherogenesis.