Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation

Table 1

Primer sequences and PCR conditions.

	Primer sequences	Denat. 𝑡/𝑇	Anneal. 𝑡/𝑇	Extens. 𝑡/𝑇	No. of cycles
𝛽-actin	sense 5^′-ATC TGG CAC CAC ACC TTC TAC AAT GAG CTG CG–3^′	1 min/94^∘C	1 min/59^∘C	1.5 min/72^∘C	25
	antisense 5^′-CGT CAT ACT CCT GCT TGC TGA TCC ACA TCT GC–3^′
IL-6	sense 5^′-ATG AAC TCC TTC TCC ACA AGC GC-3^′	1 min/94^∘C	1 min/51^∘C	1.5 min/72^∘C	30
	antisense 5^′-GAA GAG CCC TCA GGC TGG ACT G-3^′
TF	sense 5^′-ACT ACT GTT TCA GTG TTC AAG CAG TGA TTC-3^′	1 min/94^∘C	1 min/52^∘C	1.5 min/72^∘C	35
	antisense 5^′-ATT CAG TGG GGA GTT CTC CTT CCC AGC TCTG-3^′

𝑡/𝑇: time and temperature, Denat.: denaturation, Anneal.: annealing, Extens.: extension.