Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells
Figure 3
The role of free radicals in -mediated Erk2 activation and IL-8 production. (a) Mannitol (100 mM), o-phenanthroline (0.2 mM), or EGTA (2.5 mM) was added 45 minutes before direct addition of , and cells were harvested 15 minutes later for the analysis of p-Erk1/2 activity for the western blot. The inhibitors alone did not activate Erk1/2. (b) The inhibitory effects of o-phenanthroline on -induced expression (0.25 mM) of IL-8 protein secretion (ELISA) *.01 ().
