Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism
Figure 5
Expression of CCL2 mRNA in different cell populations. (a) air pouches were created and IL-4 or buffer was administered for 6 hours. Cells were harvested, pooled () and CCL2 (MCP-1) mRNA were detected as described in Materials and Methods. (b) Murine air pouches were created and the lining cells and skin cells were harvested as described in Materials and Methods. Lining cells, skin cells, murine macrophages (RAW) and epithelial Mode-K cells were stimulated for 6 hours with buffer (Ctrl) or 250 ng/ml IL-4 and the expression of CCL2 mRNA was performed by RT-PCR. Results are from one representative experiment out of at least 3. Number between parentheses represents the ratio of the signal intensity of (IL-4/18S)/Ctrl/18S) obtained after densitometry analysis using a Fluor-S multi-imager (Bio-Rad, Hercules, CA) and the Multi-Analyst version 1.1 program.
