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Mediators of Inflammation
Volume 2009, Article ID 415437, 7 pages
http://dx.doi.org/10.1155/2009/415437
Research Article

Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo

1Institute of Human Nutrition and Food Science, Molecular Nutrition, Christian-Albrechts-University of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, Germany
2Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation, Takasago, Hyogo, Japan
3Institute of Human Nutrition and Food Science, Food Science, Christian-Albrechts-University of Kiel, Hermann-Rodewald-Street 6, 24098 Kiel, Germany
4Vestische Kinder-und Jugendklinik Datteln, Universität Written/Herdecke, Dr.-Friedrich-Steiner Street 5, 45711 Datteln, Germany
5Functional Food Ingredients Division, Kaneka Corporation, Osaka, Japan

Received 27 October 2008; Revised 2 February 2009; Accepted 22 February 2009

Academic Editor: Philipp M. Lepper

Copyright © 2009 Constance Schmelzer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

MicroRNAs (miRs) are involved in key biological processes via suppression of gene expression at posttranscriptional levels. According to their superior functions, subtle modulation of miR expression by certain compounds or nutrients is desirable under particular conditions. Bacterial lipopolysaccharide (LPS) induces a reactive oxygen species-/NF-κB-dependent pathway which increases the expression of the anti-inflammatory miR-146a. We hypothesized that this induction could be modulated by the antioxidant ubiquinol-10. Preincubation of human monocytic THP-1 cells with ubiquinol-10 reduced the LPS-induced expression level of miR-146a to 78.9 13.22%. In liver samples of mice injected with LPS, supplementation with ubiquinol-10 leads to a reduction of LPS-induced miR-146a expression to 78.12 21.25%. From these consistent in vitro and in vivo data, we conclude that ubiquinol-10 may fine-tune the inflammatory response via moderate reduction of miR-146a expression.