Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo
Figure 4
Effect of ubiquinol-10 supplementation on miR-146a
expression in liver samples of LPS-treated mice. Mice were fed with ubiquinol-10 (250 mg/kg/d) or a respective control diet (no ubiquinol-10 supplement) for 7 days. Afterwards, a sublethal intraperitoneal injection of LPS (1 mg/kg BW)
was administered for further 4 hours Thereafter, liver samples were collected,
homogenized, and total RNAs including miRs were isolated as described by the manufacturer's
instructions. Subsequently, miRs were converted to cDNA and assayed by the
TaqMan-based qRT-PCR. Observed miR-146a expression levels of each animal were
related to the corresponding levels of the endogenous control (snoRNA202). Data
(two-sided -test) are means ± SEM of 6 animals in the intervention group (+QH2/+LPS) and control group (+LPS), respectively. miR-146a expression levels are
depicted as single bars of each animal (Figure 4(a)) or as total mean ± SEM of
each group (Figure 4(b)).
