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Mediators of Inflammation
Volume 2009 (2009), Article ID 417658, 6 pages
http://dx.doi.org/10.1155/2009/417658
Research Article

Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron

Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, India

Received 22 January 2009; Accepted 16 April 2009

Academic Editor: Valacchi Giuseppe

Copyright © 2009 Harsh Vardhan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent on chlamydial growth. We examined the effect of iron chelators on IL-8 production in HeLa 229 (cervix epitheloid cell, CCL2) cells infected with C. trachomatis. IL-8 production was induced by Iron chelator DFO and Mimosine, however, synergy with chlamydial infection was obtained with DFO only. Temporal expression of proinflammatory secreted cytokines IL-1beta, TNF-alpha, and IL-8 did not show synchrony in Chlamydia trachomatis infected cells. Secretion of IL-8 from Hela cells infected with C. trachomatis was not dependent on IL-1 beta and TNF- alpha induction. These results indicate towards involvement of iron in chlamydia induced IL-8 production.