Oxymatrine Downregulates TLR4, TLR2, MyD88, and NF-B and Protects Rat Brains against Focal Ischemia
Figure 3
RT-PCR results of TLR4 and NF-κB. (a) RT-PCR of TLR4 at 24 hours after the operation in each group: expression of TLR4 mRNA was evidently higher in the saline group compared with the OMT treatment group, and the difference was significant (). (b) RT-PCR of NF-κB 24 hours after the operation in each group: expression of NF-κB mRNA was similar to that of TLR4 mRNA. (c) RT-PCR of TLR4 at all time points: expression of TLR4 mRNA showed no difference among all test groups at 6 hours. It was evidently higher in the saline group at 12 hours and lasted up to 48 hours. Compared with the saline group at identical time points, expression of TLR4 mRNA was lower in the OMT treatment group, and the difference was significant and compared with Sham. (d) RT-PCR of NF-κB at all time points: expression of NF-κB mRNA was similar to that of TLR4 mRNA and compared with Sham.
