Leptin Induces an Inflammatory Phenotype in Lean Wistar Rats
Figure 3
Effects of leptin on adipose tissue, serum adiponectin, and liver. Samples of mesenteric adipose tissue, serum and liver were collected from leptin-treated rats (1 mg/kg via the tail vein) and saline controls, and the following parameters were measured: Expressions of (a) MCP-1 and (b) IL-6 in samples of mesenteric adipose tissue were assessed using predeveloped assays for real-time PCR according to the manufacturer’s instructions (Applied Biosystems). Values were calculated using a comparative method. (c) Serum levels of adiponectin were measured by ELISA. (d) Hepatic mRNA expression of ICAM-1 was measured by real-time PCR. Data are presented as mean ± SEM of at least 4 observations/group. Statistics were performed using Student’s -test P < .05 compared to saline controls.