Research Article

Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment

Figure 7

Production of cytokines by HMVEC stimulated with tumour cell conditioning medium. HMVEC cultures were exposed to 48 hours-conditioning medium of HT-29 and FaDu for the indicated periods of time. Production of cytokines by HMVEC was calculated as the difference between the value of samples and the initial amount in the conditioning mediums from tumour cells. Bars represent the mean SEM of 5 independent experiments performed by triplicate. Statistical significance was assessed using multivariate Wilks statistic and one sided Dunnet test to compare every treatment groups with the control group; , and .
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