Research Article

Linking Innate and Adaptive Immunity: Human V 9V 2 T Cells Enhance CD40 Expression and HMGB-1 Secretion

Figure 4

Change in cell surface expressed HMGB-1 (amphoterin) on adherent monocytes in PBMC following IPP and TSST-1 treatment. (a) Fluorescent microscopy of surface-expressed HMGB-1 in PBMCs. HMGB-1 is shown in green, T cells in red, and nucleus in blue. Top left panel—Resting PBMC cultured on cover slips for 48 hours were stained for surface expressed HMGB-1. Top right panel—Surface expression of HMGB-1 after 24 hour culturing in growth medium followed by 24 hour TSST-1 treatment. Bottom left panel—Surface expression of HMGB-1 48 hours after IPP treatment. (Bottom right panel) Surface expression of PBMC that had been treated overnight with IPP and subsequently treated with TSST-1 for 24 hour. (b) Bar graph of the flow cytometry analysis quantifying the release of HMGB-1 from the cell surface ( ; error bars represent SD). Statistic shown compares IPP + TSST-1 versus TSST-1 alone ( , paired Student’s -test). (c) Western blot analysis of secreted HMGB-1 in cultured supernatants.