Chemokine Receptor and Ligand Upregulation in the Diaphragm
during Endotoxemia and Pseudomonas Lung Infection
Figure 4
LPS is able to activate upregulation of the RANTES gene in cultured muscle cells. (a) RANTES mRNA levels in primary diaphragmatic myotubes under basal conditions (CTL) and in response to a 4-hour period of exposure to LPS (1 g/mL). All data are normalized to the L32 housekeeping gene. for comparisons to the control (CTL) condition. (b) RANTES promoter activation as reflected by luciferase activity in C2C12 muscle cells previously transfected with a RANTES promoter-luciferase reporter gene plasmid. Myotubes were again analyzed under basal conditions (CTL) and following a 4-hour period of stimulation with LPS (1 g/mL). for comparisons to the control (CTL) condition.
