Eicosanoids in the Innate Immune Response: TLR and Non-TLR Routes
Figure 4
Coimmunoprecipitation of dectin-1 and DC-SIGN. DC were incubated in the presence and absence of 1 mg/ml zymosan for 10 minutes and then the cell lysates were used for immunoprecipitation with either 5 g anti-DC-SIGN or 5 g anti-glutathione S-transferase (GST) control mAb and blotting with anti-DC-SIGN, anti-dectin-1, or anti-GST Ab (a). HEK293 cells were transfected with the indicated expression vectors and the cell lysates immunoprecipitated with either 2 g anti-DC-SIGN or 0.5 g anti-Myc mAb (b). Upper panels show the expression of the Myc tag and DC-SIGN in the immunoprecipitates. The lower panels show the expression of the proteins in the cell lysates. Cells transfected with pEF4 empty vector were used where no expression vector is indicated. Immunoprecipitations have been conducted with 2 mg protein per condition. The lanes corresponding to cell lysates were loaded with 40 g protein. Reproduced with permission [53].
