Effect of Akt inhibitor, SH-5 (SH), on ghrelin- (Gh-) induced cNOS phosphorylation in gastric mucosal cells exposed to H. pylori LPS. The cells were treated with Gh (0.5 g/mL) or SH (20 M) + Gh and incubated for 16 h in the presence of 100 ng/ml LPS. Cell lysates were resolved on SDS-PAGE, transferred to nitrocellulose, and probed with phosphorylation-specific cNOS (pcNOS) antibody, and after stripping reprobed with anti-cNOS antibody. The immunoblots shown are representative of three experiments.
