Mediators of Inflammation / 2010 / Article / Fig 7

Research Article

Ghrelin Protection against Lipopolysaccharide-Induced Gastric Mucosal Cell Apoptosis Involves Constitutive Nitric Oxide Synthase-Mediated Caspase-3 S-Nitrosylation

Figure 7

Effect of Akt inhibitor, SH-5 (SH), on ghrelin- (Gh-) induced cNOS phosphorylation in gastric mucosal cells exposed to H. pylori LPS. The cells were treated with Gh (0.5 μg/mL) or SH (20 μM) + Gh and incubated for 16 h in the presence of 100 ng/ml LPS. Cell lysates were resolved on SDS-PAGE, transferred to nitrocellulose, and probed with phosphorylation-specific cNOS (pcNOS) antibody, and after stripping reprobed with anti-cNOS antibody. The immunoblots shown are representative of three experiments.

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