Research Article

Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage?

Figure 5

Release of danger signals during ischemic storage of liver. Effluent obtained after flushing the liver in half-hourly or hourly intervals for either 24 or 6 h was assessed for HMGB1 and MIF using Western blot (a). Release of both danger signals, HMGB1 and MIF, was detected by Western blot. (a1) Cold ischemia; (a2) mechanical stress plus cold ischemia; (a3) warm ischemia; (a4) mechanical stress plus warm ischemia. Band density was assessed using Image J program and expressed as ng/ml (HMBG1) or arbitrary units (MIF) (b). Danger signals were released significantly earlier in both groups subjected to mechanical stress compared with ischemia only group ( 𝑃 = . 0 0 2 7 ; 𝑃 = . 0 0 2 , resp.). At earlier time points (0.75–1.5 h/5–11 h in warm/cold ischemic ischemia), the relative concentration of HMGB1 and MIF in the effluent was also significantly higher compared with groups subjected to ischemia only ( 𝑃 < . 0 5 ).
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(a)
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(b)