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Mediators of Inflammation
Volume 2010, Article ID 482950, 6 pages
http://dx.doi.org/10.1155/2010/482950
Clinical Study

Polymorphisms in Tumour Necrosis Factor Alpha (TNF ) Gene in Patients with Acute Pancreatitis

1Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey
2Department of General Surgery, Faculty of Medicine, Istanbul University, 34390 Istanbul, Turkey

Received 26 September 2009; Revised 13 December 2009; Accepted 25 February 2010

Academic Editor: Giuseppe Valacchi

Copyright © 2010 Gül Özhan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Proinflammatory cytokines, such as tumour necrosis factor (TNF ), play fundamental roles in the pathogenesis of acute pancreatitis (AP). The aim of this study was to determine if polymorphisms in the TNF gene are associated with AP. Two polymorphisms located in the promoter region (positions and ) in TNF gene were determined using polymerase chain reaction- (PCR-) restriction fragment length polymorphism (RFLP) methods in 103 patients with AP and 92 healthy controls. Odds ratios (ORs) and 95% confidence intervals (CI) were estimated using logistic regression analysis adjusted for age, sex, BMI and smoking. The frequencies of TNF polymorphisms were both similar in patients with mild or severe pancreatitis, so were in pancreatitis patients and in controls. We suggest that both SNPs of TNF are not genetic risk factor for AP susceptibility (OR 1.63; 95% CI: 1.13−4.01 for TNF and OR 0.86; 95% CI: 0.75−1.77 for TNF ).