Peritoneal Adipocytes and Their Role in Inflammation during Peritoneal Dialysis
Figure 1
Constitutive expression of mRNA (expressed as amplicon ratio after normalized to GAPDH, measured by quantitative PCR), protein for G-protein-coupled chemokine receptor (CXCR4; expressed as ratio of densitometry data after normalized to GAPDH, measured by immunoblotting), and stromal derived factor-1 (SDF-1; measured by ELISA) in cultured human peritoneal mesothelial cells (HPMC), stromal vascular fraction (SVF), and adipocytes from human omental tissue (a to d). The CXCR4 expression in HPMC was up-regulated in a dose-dependent manner with hepatocyte growth factor (HGF) after 4 hours culture (e and f). Overnight PD effluent fluid ( = 15) was collected from CAPD patients on day 28 after the onset of peritonitis. Control PD effluent fluid ( = 15) was obtained in CAPD patients without previous history of peritonitis. The concentration of the HGF in PD effluent fluid was measured by ELISA. Persistent release of HGF in PD effluent was observed at day 28 after peritonitis in CAPD patients (g). These data are from our unpublished studies.
