The p110𝛼 and p110𝛽 Isoforms of Class I Phosphatidylinositol 3-Kinase Are Involved in Toll-Like Receptor 5 Signaling in Epithelial Cells

Figure 4

PI3K inhibition reduces IL-8 production after flagellin stimulation in Caco-2 cells. Caco-2 cells seeded as described in Methods were treated with the inhibitors shown (all at 10 $\mu$M, except for 30 $\mu$M LY29 and LY30) or an equivalent dose of DMSO vehicle in complete culture media. Thirty minutes later, flagellin was added to a final concentration of 100 ng/ml, and cell supernatants harvested 3 hours later. IL-8 was measured by ELISA, and the values in each experiment were normalized to the flagellin + DMSO wells. (a) Effects of broadly reactive PI3K inhibitors. (b) Effects of isoform-specific inhibitors. ${}^{*}P<.001$ versus DMSO; ${}_{}{}^{**}P<.01$ versus DMSO (t-test). Results compiled from N of at least 10 for each inhibitor.

(a)

(b)