Mediators of Inflammation / 2010 / Article / Fig 3

Research Article

Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells

Figure 3

IFN-γ production in response to HMB-PP is restricted to γδ T cells and is highest 72 hours after simulation. Freshly isolated PBMCs were enriched for αβ and γδ T cells using magnetic beads, and 200,000 cells of each population were stimulated with various concentrations of HMB-PP (a and c) or IPP (b). The levels of IFN-γ in cell supernatants were quantified by ELISA after 24 hours (a and b) or 72 hours (c). Results are expressed as mean (± SEM) levels of IFN-γ released by αβ and γδ T cells from 2–8 donors. (d) Mean (± SEM) IFN-γ production by γδ T cells from 8 donors after stimulation with 10 μM IPP, 10 nM HMB-PP, 50 U/mL IL-2, and/or 10 ng/mL IL-15 as indicated. * 𝑃 < . 0 5 compared to unstimulated cells (medium). ns: not significant comparing absence and presence of IL-2 and IL-15.

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