Mediators of Inflammation / 2010 / Article / Fig 4

Research Article

Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells

Figure 4

Phosphoantigen stimulation of γδ T cells causes no detectable increase in IL-10, IL-13, or IL-17 production. Freshly isolated PBMCs were enriched for γδ T cells using magnetic beads, and 200,000 cells were stimulated with 10 μM IPP, 10 nM HMB-PP, 50 U/mL IL-2, and/or 10 ng/mL IL-15 as indicated. The levels of IL-10 (a), IL-13 (b), and IL-17 (c) in cell supernatants were measured by ELISA after 72 hours. Bar charts show mean cytokine levels for all treatments for 8 donors. ns: not significant comparing absence and presence of IL-2 and IL-15. PMA/I-PBMC, cytokine levels released by PMA and ionomycin-stimulated PBMC.

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