Expression of TLR4-MyD88 and NF-𝜅B in the Iris during Endotoxin-Induced Uveitis

Figure 4

Immunohistochemistry of TLR4 (DAB): (a) TLR4 could not be detected in the iris at 0 h. (b) The TLR4 located adjacent to blood vessels in the iris at 12 h. (c) Most of TLR4 expressed in the iris at 24 h. (d) The TLR4 positive cells were shown at 48 h. (e) TLR4 positive cells had been decreased in the iris at 72 h ($\text{Bar}=20$ $\mu$m). (f) No positive staining was observed in the iris with a nuclear counterstain when under identical experimental conditions when replacing of the primary antibody with normal rabbit IgG at the same concentration (negative control, $\text{Bar}=10$ $\mu$m).

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