Leptin Administration Downregulates the Increased Expression Levels of Genes Related to Oxidative Stress and Inflammation in the Skeletal Muscle of ob/ob Mice

Table 2

Total body and skeletal muscle weights and biochemical characteristics of wild type and ob/ob mice.

	wild type	control ob/ob	pair-fed ob/ob	leptin-treated ob/ob
Body weight (g)	$25.6\pm 0.3$	$47.8\pm {4.9}^{\text{b}}$	$35.7\pm 0.7$	$24.7\pm {1.2}^{\text{d},\text{f}}$
Gastrocnemius (mg)	$142.9\pm 3.4$	$90.7\pm {10.0}^{\text{b}}$	$68.5\pm 1.6$	$104.9\pm {2.6}^{\text{b},\text{f}}$
Gastrocnemius (mg/g)	$5.59\pm 0.12$	$1.91\pm {0.11}^{\text{b}}$	$1.92\pm 0.07$	$4.28\pm {0.15}^{\text{b},\text{d},\text{f}}$
Glucose (mg/dL)	$149\pm 42$	$430\pm {59}^{\text{a}}$	$160\pm {24}^{\text{d}}$	$178\pm {29}^{\text{d}}$
FFA (mmol/L)	$1.62\pm 0.49$	$1.61\pm 0.30$	$1.65\pm 0.12$	$0.78\pm {0.13}^{\text{c},\text{f}}$
Glycerol (mmol/L)	$42.8\pm 6.7$	$81.6\pm {19.6}^{\text{a}}$	$39.6\pm {4.9}^{\text{c}}$	$12.3\pm {4.7}^{\text{a},\text{d},\text{f}}$
TG (mg/dL)	$122\pm 18$	$169\pm 32$	$151\pm 10$	$86\pm {17}^{\text{e}}$
Insulin (ng/mL)	$0.42\pm 0.09$	$8.60\pm {1.51}^{\text{b}}$	$2.40\pm {0.68}^{\text{c}}$	$0.47\pm {0.09}^{\text{d},\text{e}}$
Adiponectin ($\mu$g/mL)	$30.2\pm 3.0$	$28.3\pm 5.4$	$39.1\pm 1.8$	$40.2\pm 3.0$
Leptin (ng/mL)	$1.36\pm 0.42$	UD	UD	$3.48\pm 1.02$
HOMA	$4.3\pm 1.8$	$202.4\pm {33.8}^{\text{b}}$	$25.8\pm {10.4}^{\text{d}}$	$5.12\pm {1.1}^{\text{d}}$
QUICKI	$0.333\pm 0.023$	$0.205\pm {0.003}^{\text{b}}$	$0.263\pm {0.015}^{\text{d}}$	$0.311\pm {0.016}^{\text{d}}$

Data are $\text{mean}\pm \text{SEM}$ ($n=5$ per group). Differences between groups were analyzed by Kruskal-Wallis followed by Mann Whitney’s U test. ${}^{\text{a}}P<.05$ and ${}^{\text{b}}P<.01$ versus wild type. ${}^{\text{c}}P<.05$ and ${}^{\text{d}}P<.01$   versus ob/ob. ${}^{\text{e}}P<.05$ and ${}^{\text{f}}P<.01$ versus pair-fed ob/ob. FFA: free fatty acids. TG: triglycerides. UD: undetectable. HOMA: homeostasis model assessment. QUICKI: quantitative insulin sensitivity check index.