Leptin Administration Downregulates the Increased Expression Levels of Genes Related to Oxidative Stress and Inflammation in the Skeletal Muscle of ob/ob Mice

Table 5

Genes involved in oxidative stress and inflammatory responses altered by leptin in the gastrocnemius muscle of ob/ob mice.

GeneBank Number	Gene Symbol	Gene Name	Fold change		Ratio
			ob/ob	leptin
Genes downregulated by leptin
NM_009804	Cat	Catalase	1.47	1.13	0.77
NM_007705	Cirbp	Cold inducible RNA binding protein	1.68	1.14	0.68
NM_009964	Cryab	Crystallin, $\alpha$ B	1.32	1.15	0.87
NM_023695	Crybb1	Crystallin, $\beta$ B1	2.21	1.39	0.63
NM_023646	Dnaja3	DnaJ (Hsp40) homolog, subfamily A, member 3	0.95	0.64	0.67
NM_021422	Dnaja4	Heat shock protein, DNAJ-like 4	0.88	0.30	0.34
NM_018808	Dnajb1	DnaJ (Hsp40) homolog, subfamily B, member 1	0.44	0.33	0.74
NM_026400	Dnajb11	DnaJ (Hsp40) homolog, subfamily B, member 11	1.11	0.93	0.84
NM_027287	Dnajb4	DnaJ (Hsp40) homolog, subfamily B, member 4	1.09	0.60	0.55
NM_019874	Dnajb5	DnaJ (Hsp40) homolog, subfamily B, member 5	1.03	0.73	0.72
NM_011847	Dnajb6	DnaJ (Hsp40) homolog, subfamily B, member 6 isoform c	0.70	0.47	0.67
NM_013760	Dnajb9	DnaJ (Hsp40) homolog, subfamily B, member 9	0.62	0.39	0.63
NM_007869	Dnajc1	DnaJ (Hsp40) homolog, subfamily C, member 1	0.82	0.52	0.63
NM_028873	Dnajc14	DnaJ (Hsp40) homolog, subfamily C, member 14	1.12	0.87	0.77
NM_172338	Dnajc16	DnaJ (Hsp40) homolog, subfamily C, member 16	1.15	0.66	0.57
NM_009584	Dnajc2	DnaJ (Hsp40) homolog, subfamily C, member 2	1.01	0.82	0.81
NM_008929	Dnajc3	DnaJ (Hsp40) homolog, subfamily C, member 3B	1.02	0.83	0.82
NM_016775	Dnajc5	DnaJ (Hsp40) homolog, subfamily C, member 5	0.74	0.50	0.67
NM_010344	Gsr	Glutathione reductase 1	1.17	0.71	0.61
NM_008180	Gss	Glutathione synthetase	1.13	0.88	0.78
NM_010357	Gsta4	Glutathione S-transferase, $\alpha$ 4	1.50	1.46	0.97
NM_010362	Gsto1	Glutathione S-transferase ο 1	1.42	1.15	0.81
NM_008198	H2-Bf	Histocompatibility 2, complement component factor B	2.00	1.44	0.72
NM_013558	Hspa1l	Heat shock 70kDa protein 1-like	1.60	1.04	0.65
NM_008301	Hspa2	Heat shock protein 2	1.49	0.98	0.65
NM_008300	Hspa4	Heat shock protein 4	0.92	0.30	0.32
NM_031165	Hspa8	Heat shock protein 8	0.91	0.57	0.62
NM_010481	Hspa9a	Heat shock protein 9	1.03	0.88	0.86
NM_024441	Hspb2	Heat shock protein 2	1.45	1.21	0.83
NM_019960	Hspb3	Heat shock protein 3	1.66	1.27	0.77
NM_013868	Hspb7	Heat shock protein family, member 7	1.83	0.35	0.19
NM_008302	Hspcb	Heat shock protein 1, $\beta$	0.86	0.69	0.80
NM_008416	Junb	Jun-B oncogene	0.59	0.36	0.61
NM_010592	Jund1	Jun D proto-oncogene	1.49	0.94	0.63
NM_008456	Klk5	Kallikrein 5	2.23	1.43	0.64
NM_026346	MAFbx	Muscle atrophy F box	0.65	0.43	0.67
NM_008209	Mr1	Histocompatibility-2 complex class 1-like	1.19	0.98	0.82
NM_008630	Mt2	Metallothionein 2	1.11	0.50	0.46
NM_008631	Mt4	Metallothionein 4	1.27	1.03	0.81
NM_008872	Plat	Plasminogen activator, tissue	1.56	1.12	0.72
NM_029397	Rbm12	RNA binding motif protein 12	1.40	1.03	0.74
NM_026453	Rbm13	RNA binding motif protein 13	1.01	0.87	0.86
NM_026434	Rbm18	RNA binding motif protein 18	0.94	0.59	0.63
BC080205	Rbm22	RNA binding motif protein 22	1.14	0.75	0.66
BC040811	Rbm28	Rbm28 protein	0.69	0.49	0.71
NM_172762	Rbm34	RNA binding motif protein 34	1.01	0.67	0.66
NM_009032	Rbm4	RNA binding motif protein 4	1.04	0.81	0.78
NM_148930	Rbm5	RNA binding motif protein 5	0.69	0.63	0.91
NM_144948	Rbm7	RNA binding motif protein 7	0.81	0.74	0.91
NM_025875	Rbm8a	RNA binding motif protein 8a	0.91	0.69	0.76
NM_175387	Rbm9	RNA binding motif protein 9 isoform 2	1.96	0.46	0.23
NM_025429	Serpinb1a	Serine (or cysteine) proteinase inhibitor, clade B, member 1a	2.73	2.09	0.77
NM_080844	Serpinc1	Serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1	4.98	1.93	0.39
NM_008871	Serpine1	Serine (or cysteine) proteinase inhibitor, clade E, member 1	2.12	0.97	0.46
NM_011340	Serpinf1	Serine (or cysteine) proteinase inhibitor, clade F, member 1	2.43	1.50	0.62
NM_009776	Serping1	Serine (or cysteine) proteinase inhibitor, clade G, member 1	1.41	1.15	0.81
NM_009776	Serping1	Serine (or cysteine) proteinase inhibitor, clade G, member 1	1.41	1.15	0.81
NM_013749	Tnfrsf12a	Tumor necrosis factor receptor superfamily, member 12a	0.78	0.29	0.37
Genes upregulated by leptin
NM_030004	Cryl1	Crystallin $\lambda$ 1	1.25	1.72	1.38
NM_016669	Crym	Crystallin $\mu$	1.37	1.64	1.19
NM_133679	Cryzl1	Crystallin, $\zeta$ (quinone reductase)-like 1	1.10	1.28	1.16
NM_008161	Gpx3	Glutathione peroxidase 3 isoform 2	0.47	0.54	1.15
NM_024198	Gpx7	Glutathione peroxidase 7	1.00	1.34	1.33
NM_010359	Gstm3	Glutathione S-transferase, $\mu$ 3	1.06	1.23	1.17
NM_010360	Gstm5	Glutathione S-transferase, $\mu$ 5	1.09	1.39	1.27
NM_013541	Gstp1	Glutathione S-transferase, $\pi$ 1	0.87	1.04	1.20
NM_010361	Gstt2	Glutathione S-transferase, $\theta$ 2	1.21	1.70	1.40
NM_133994	Gstt3	Glutathione S-transferase, $\theta$ 3	1.53	1.69	1.11
NM_010363	Gstz1	Glutathione transferase zeta 1 (maleylacetoacetate isomerase)	1.13	1.24	1.10
NM_010378	H2-Aa	Histocompatibility 2, class II antigen A, $\alpha$	0.46	1.26	2.76
NM_010379	H2-Ab1	Histocompatibility 2, class II antigen A, $\beta$ 1	0.37	1.04	2.84
NM_010382	H2-Eb1	Histocompatibility 2, class II antigen E $\beta$	0.43	1.03	2.40
NM_010395	H2-T10	Histocompatibility 2, T region locus 10	1.11	1.41	1.27
NM_013559	Hsp105	Heat shock protein 105	0.41	0.73	1.79
NM_008303	Hspe1	Heat shock protein 1 (chaperonin 10)	0.67	0.98	1.48
AK_052911	MuRF1	M muscle RING finger 1	0.20	0.28	1.43
XM_131139	Rbm15	RNA binding motif protein 15	0.81	1.34	1.66
NM_197993	Rbm21	RNA binding motif protein 21	0.67	0.73	1.08
BC029079	Rbm26	Rbm26 protein	0.75	1.19	1.59
AK087759	Rbm27	RNA binding motif protein 27	0.88	1.19	1.36
NM_148930	Rbm5	RNA binding motif protein 5	0.77	1.18	1.55
NM_011251	Rbm6	RNA binding motif protein 6 isoform a	0.80	0.97	1.21
NM_207105	Rmcs1	histocompatibility 2, class II antigen A, $\beta$ 1	0.38	0.89	2.37
NM_011454	Serpinb6b	Serine (or cysteine) proteinase inhibitor, clade B, member 6b	1.06	1.23	1.16
NM_009825	Serpinh1	Serine (or cysteine) proteinase inhibitor, clade H, member 1	0.65	0.99	1.53
NM_145533	Smox	Spermine oxidase	0.41	1.23	3.00
AK080908	Sod1	Superoxide dismutase	0.58	0.62	1.07
NM_011723	Xdh	Xanthine dehydrogenase	0.68	1.01	1.47

Differential expression of genes is indicated as fold changes with respect to the wild type group presenting only the genes which were significantly different ($P<.05$) between the leptin-treated and the ob/ob groups. Ratio: fold change value for leptin-treated between the ob/ob groups.