Research Article

The Traditional Japanese Formula Keishibukuryogan Inhibits the Production of Inflammatory Cytokines by Dermal Endothelial Cells

Figure 2

Effect of paeoniflorin on LPS-stimulated induction of cytokines in HDMECs. (a) HDMEC cells that were treated with LPS (1 μg/mL) and/or paeoniflorin (100 μg/mL) for 6 or 24 h and were subjected to the MTT assay. (b) HDMEC cells were treated with 1 μg/mL LPS and/or 100 μg/mL of paeoniflorin for 24 h. The MIF, IL-6, IL-8, and TNF-α protein content of cultured supernatants was analyzed by ELISA. Data are presented as the means ± S.D (n = 5). *P  <  .0005. (c) The cells were treated with 1 μg/mL LPS and/or 100 μg/mL of paeoniflorin for 6 h. Total RNA was isolated, and the mRNA expression levels of MIF, IL-6, IL-8, and TNF-α were detected by RT-PCR. (These experiments were repeated three times with similar results).
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