Mediators of Inflammation / 2010 / Article / Fig 4

Research Article

Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells

Figure 4

Inhibitors of ERK, NF-κB, and PI3K blocked the CD147-mediated activation of IFITM1 expression. (a) THP-1 cells were preincubated with 10 μM of U0126 (U), 5 μM of TPCK (T), 0.2% of DMSO (D), 10 μM of JNK inhibitor (JI), negative control of JNK inhibitor (J(-)) for 30 min, and 20 μM of LY294002 (LY) or 1 μM of Ro-31-8425 (Ro) for 60 min and then stimulated with 10 μg/mL of anti-CD147 mAb for 48 hr. Cell lysates were collected and subjected to Western blot analysis using mAbs specific to either IFITM1 or actin. (b) THP-1 cells were stimulated with 10 μg/mL of anti-CD147 mAb or mouse IgG (mIgG) for indicated times. Cell lysates were collected and subjected to Western blot analysis using mAbs specific to either AKT or phosphor-AKT. These experiments were repeated twice with essentially the same results.
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