Distribution of Major Basic Protein on Human Airway following In Vitro Eosinophil Incubation

Figure 7

Overnight incubation of human airway with exogenously applied MBP. This representative image shows fluorescence of immunolabeled MBP (red) in human airway following incubation with MBP 10^-5 M overnight. Compared to lesser duration of exposure (Figure 6), longer exposure, overnight, allowed MBP greater access to cells within the media of the airway wall. Notably this longer exposure allowed for significant immunolocalization to be detected within the smooth muscle layer (green) (confocal microscopy 400x).