Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines
C. parapsilosis adhesion to the gingival epithelial monolayer culture. Human gingival cells were grown up to 80% confluence, pulsed with C. parapsilosis or C. albicans, and incubated for 6 hours with or without serum. Following this culture period, the supernatant containing nonattached Candida was discarded, and the cultures were washed and stained with Cristal violet. Representative photographs are presented. Magnification: 250.