Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines
Quantification of HBD2 and HBD3 levels secreted by gingival epithelial cells following infection with C. parapsilosis. Gingival epithelial cells were cultured with or without C. parapsilosis for 6 and 24 hours. Supernatants were collected and used to quantify HBD2 (a) and HBD3 (b) using ELISA kits. Data are means ± SD of three separate experiments. The levels of significance were obtained by comparing the amount of AMP obtained with unstimulated samples to that obtained with samples stimulated C. parapsilosis.