Mediators of Inflammation / 2010 / Article / Fig 8

Research Article

Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines

Figure 8

Quantification of mRNA expression levels of IL-1β, TNFα, and IFNγ by gingival epithelial cells following infection with C. parapsilosis. Gingival epithelial cells were cultured with or without C. parapsilosis for 6 and 24 hours. Total RNA was extracted from the cells following each culture period and was used for quantitative RT-PCR of the IL-1β, TNFα, and IFNγ genes, as described in Section 2. Results are presented as a fold expression of the gene in the test sample compared to this gene expression in the control. Data are expressed as means ± SD from triplicate assays of three different experiments. (a) IL-1β expression, (b) TNFα   expression, and (c) IFNγ expression. GEC: gingival epithelial cells.