Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines

Figure 8

Quantification of mRNA expression levels of IL-1$\beta$, TNF$\alpha$, and IFN$\gamma$ by gingival epithelial cells following infection with C. parapsilosis. Gingival epithelial cells were cultured with or without C. parapsilosis for 6 and 24 hours. Total RNA was extracted from the cells following each culture period and was used for quantitative RT-PCR of the IL-1$\beta$, TNF$\alpha$, and IFN$\gamma$ genes, as described in Section 2. Results are presented as a fold expression of the gene in the test sample compared to this gene expression in the control. Data are expressed as means ± SD from triplicate assays of three different experiments. (a) IL-1$\beta$ expression, (b) TNF$\alpha \mathrm{\hspace{0.17em}\hspace{0.17em}}$ expression, and (c) IFN$\gamma$ expression. GEC: gingival epithelial cells.

(a)

(b)

(c)