Mediators of Inflammation / 2010 / Article / Tab 1

Research Article

Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines

Table 1

Primer sequences used for the q-RT-PCR.

Gene namePrimers sequencesProduct size (bp)

TLR-2sense: 5-GCCTCTCCAAGGAAGAATCC-3144
antisense: 5-TCCTGTTGTTGGACAGGTCA-3

TLR-4sense: 5-AATCTAGAGCACTTGGACCTTTCC-3116
antisense: 5-GGGTTCAGGGACAGGTCTAAAGA-3

TLR-6sense: 5-CATCCTATTGTGAGTTTCAGGCAT-3121
antisense: 5-GCTTCATAGCACTACATCCCAAG-3

TLR-9sense: 5-GGACCTCTGGTACTGCTTCCA-3151
antisense: 5-AAGCTCGTTGTACACCCAGTCT-3

β1-defensinsense: 5-GCCTCTCCCCAGTTCCTGAA-382
antisense: 5-GCAGAGAGTAAACAGCAGAAGGTA-3

β2-defensinsense: 5-TGTGGTCTCCCTGGAACAAAAT-3105
antisense: 5-GTCGCACGTCTCTGATGAGG-3

β3-defensinsense: 5-CTTCTGTTTGCTTTGCTCTTCCT-3138
antisense: 5-CTGTTCCTCCTTTGGAAGGCA-3

β4-defensinsense: 5-CACTCTACCAACACGCACCTAG-3133
antisense: 5-CGCAACTGGAACCACACACT-3

IL-1βsense: 5-CTGTCCTGCGTGTTGAAAGA-369
antisense: 5-TTGGGTAATTTTTGGGATCTACA-3

IFNγsense: 5-GGCATTTTGAAGAATTGGAAAG-3111
antisense: 5-TTTGGATGCTCTGGTCATCTT-3

TNFαsense: 5-CAGCCTCTTCTCCTTCCTGAT-3122
antisense: 5-GCCAGAGGGCTGATTAGAGA-3

GAPDHsense: 5-GGTATCGTCGAAGGACTCATGAC-3180
antisense: 5-ATGCCAGTGAGCTTCCCGTTCAGC-3