Regulatory Effect of Melatonin on Cytokine Disturbances in the Pristane-Induced Lupus Mice

Figure 2

All mice were sacrificed at the end of 24 weeks, and splenic lymphocytes were seeded at $1\times {10}^6$ cells/well. IL-2 concentrations in splenic lymphocytes were stimulated for 48 h with 3 mg/L ConA. IL-6, and IL-13 concentrations in splenic lymphocytes were stimulated for 48 h with 12 mg/L LPS. IL-2, IL-6, and IL-13 concentrations in culture supernatants were detected by ELISA. Data were given in $\text{mean}$$\pm$ SD ($n=4\text{–}5$). ${}_{}{}^{**}P<.01,$${}_{}{}^{*}P<.05$ versus Normal control group, $∆P<.05\text{,}$$∆∆P<.01$ versus Model control group.

(a)

(b)

(c)