Lipopolysaccharide Inhibits the Channel Activity of the P2X7 Receptor

Figure 1

LPS inhibits ATP-induced currents in P2X7R-HEK293 cells. (a) LPS alone (1 μg/mL) does not gate currents nor inhibit 300 μM ATP-gated currents when coapplied. Data are representative of 5-6 independent experiments, in the absence of FBS. (b) Representative experiment of the LPS-induced inhibition of ATP-evoked currents. The effect of LPS is time-dependent, experiments were run in the presence of 1% FBS. (c) Summary of the effect of LPS on ATP-evoked currents after 1, 10, and 15 min of preapplication (𝑛=6-8, 𝑃<0.05, Kruskal-Wallis test). (d) ATP concentration-response curves in the absence (open circles) or in the presence of 1 μg/mL LPS (closed circles). LPS was added 30 min before the start of each experiment (𝑛=5).

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