Lipopolysaccharide Inhibits the Channel Activity of the P2X7 Receptor
LPS does not change the permeability of the P2X7R. (a) Voltage-ramp protocols were applied with 300 μM ATP alone (grey line) or treated with 1 μg/mL LPS (black line); the insert shows a closeup of reversal potentials. Control and treated experiments were performed in parallel and repeated independently 4 times. (b) The same protocol was performed in the presence of 300 μM ATP (ATP), preincubated with LPS (ATP + LPS) with normal and 10 mM extracellular calcium (A + L + 10 mM Ca2+).