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Mediators of Inflammation
Volume 2011 (2011), Article ID 152625, 12 pages
Research Article

Lipopolysaccharide Inhibits the Channel Activity of the P2X7 Receptor

1Centro Fondap de Estudios Moleculares de la Célula Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Chile
2Section on Cellular Signaling, Program on Developmental Neuroscience (PDN), NICHD, Bethesda, Md, USA
3Departamento de Biología, Facultad de Química y Biología y Centro de Biotecnología Acuícola, Universidad de Santiago de Chile (USACH), Chile
4Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Chile
5Instituto de Química, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso PUCV, Avenida Universidad 330, Curauma, Valparaíso, Chile
6Centro de Regulación Celular y Patología J.V. Luco y Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Chile
7Departamento de Ciencias Básicas y Comunitarias, Facultad de Odontología, Universidad de Chile, Chile
8Emory Black, Facultad de Ciencias Médicas, Universidad de Santiago de Chile (USACH), Chile

Received 29 March 2011; Revised 3 June 2011; Accepted 20 June 2011

Academic Editor: Dennis Daniel Taub

Copyright © 2011 Elias Leiva-Salcedo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Sup. Fig. 1: HEK293 cells do not express the TLR-4receptor. PCR were done for TLR4 and RNAr 16S has a housekeeping gene. Reactions obtained were electrophorized in agarosa gel and visualized by UV staining in transilluminator.

Sup. Fig. 2: P2Y have lower contribution in ATP inducing calcium movement. Representative recordings showing wild-type (upper panel) and P2X7R-expressing (lower panel) HEK293 cells. In the complete absence of extracellular calcium (by the addition of 5 mM EGTA), no responses were observed. These results were observed in at least 3 different cells.

Sup. Fig. 3: LPS alone cannot trigger ERK activation in P2X7R-HEK293 cells. (A) Representative gel showing ERK activation is induced by 1 mM ATP and the lack of effect of LPS alone (1 µg/mL) applied at different times. (B) LPS-induced activation of ERK in Raw 264.7 cells; these cells express the classical LPS receptor TLR-4. In both cases upper panels show the detection of P-ERK and lower panels show total ERK.

  1. Supplementary Figures
  2. Supplementary Material