Phosphodiesterase Type 4D Gene Polymorphism: Association with the Response to Short-Acting Bronchodilators in Paediatric Asthma Patients

Table 1

Primers used for amplification and genotyping of PDE4D polymorphisms.

SNP ID	Position	PCR primers	ASO primers
rs1544791	Intron 2	GAAGCATGTTGCACATAAACTG,	ATGTGGCA/GTTATTTGC
		TCTCTTCTACTCTATGGGCTTTCTG
rs1504982	−1345	GGGAGCTACAAAATGCATCC,	ATTCCTTGC/TCTTTTAAA
		CAAGTGGTTGACCCTGTCCT
rs10940648	−984	CGTGATTTCCTTTATTGCCTTT,	AGTAAAAAT/CGTTGTAAC
		TTCCCTATGCAAAGGTTTGAG
rs829259	3′UTR	TTGTCCCTGAGTGAAGTCTAGAAA,	TCCCTACT/ACTTAGTAT
		CCCTTTCAGGTCTGGATTTG

rs1544791 is located in intron 2 of PDE4D7 isoform; positions of rs1504982 and rs10940648 refer to the start of PDE4D5 mRNA (AF012073) and, rs829259 is located in 3′UTR, common to all splice variants.