Immunomodulatory Effects of Aerobic Training in Obesity
Figure 2
Expression of TLR-2 (a), TLR-4 (b), and TLR-7 (c) by analysis of mRNA levels via RT-PCR. Training-induced changes were analyzed using the delta-delta ct method. Expression of MyD88 (d), TLR-2 (e), TLR-4 (f), and TLR-7 (g) by analyses of protein expression (Western blot of 4 representative examples of the LNE-group). Densitometry analysis (h) found a significant increased expression for TLR-4 and TLR-7. For MyD88 and TLR-2, no significant changes were found. Statistical analysis of the different time-points (intra group-analysis) was performed using the Wilcoxon-Test; the comparison of the different groups with each other (inter group analysis) was done by using the Mann-Whitney Test (**; *; for PCR and for Western blot).
