Surface TLR2 and TLR4 Expression on Mature Rat Mast Cells Can Be Affected by Some Bacterial Components and Proinflammatory Cytokines
Figure 2
The effect of LPS, LAM and PGN on rat mast cell cysLT synthesis. (a) Mast cells were preincubated with cDMEM (control) or anti-TLR antibodies for 15 min and after washing incubated with LPS, LAM, PGN or buffer alone (spontaneous cysLT generation) for 60 min. Results are expressed as the mean ± SEM of three independent experiments and each experiment was done in duplicate . (b) Mast cells were preincubated with cDMEM (control) or MG-132 for 15 min and after washing incubated with LPS, LAM, PGN or buffer alone (spontaneous cysLT generation) for 60 min. Results are expressed as the mean ± SEM of three independent experiments and each experiment was done in duplicate . **.
