Surface TLR2 and TLR4 Expression on Mature Rat Mast Cells Can Be Affected by Some Bacterial Components and Proinflammatory Cytokines
Figure 6
The effect of (a) TNF, (b) IL-6, and (c) CCL5 on TLR4 expression on rat mast cells. Mast cells were incubated with cytokines or cDMEM (control TLR4 expression on unstimulated cells referred to as 100%) for 1, 3, 6, 12, or 24 hours. Cytokine concentrations −1 pg/mL (dark bars) and 102 pg/mL (bright bars). Results are presented as a percentage of control TLR2 expression. Each point represents the mean ± SEM of 6 independent experiments. **. (d) Representative flow cytometry histogram showing TLR4 expression on rat mast cells treated with 1 pg/mL IL-6 for 3 h. (e) Representative flow cytometry histogram showing TLR4 expression on rat mast cells treated with 1 pg/mL CCL5 for 14 h. Filled histograms: untreated mast cells (control) and open histograms: IL-6- or CCL5-treated mast cells.
