Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils
Figure 1
Effects of LPS and 1, 25-vit D3 on expression of VDR, 1-α hydroxylase, COX-2, and 5-LOX in neutrophils. Adult (black column) and neonatal (grey column) neutrophils were incubated with LPS (1 g/mL) and/or 1,25-Vit D3 (10−7 M), or medium control for 4 h. mRNA expression of the (a) VDR, (b) 1-α hydroxylase, (c) COX-2, and (d) 5-LOX. Results were normalized to GAPDH expression. Each bar represents the mean ± SE (–11). *Significantly different () from LPS; **significantly different () from control; †significantly different () from adult.
