Research Article

Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils

Figure 2

Effects of MEHP on inflammatory mediator production. Adult (black column) and neonatal (grey column) neutrophils were incubated in the presence or absence of LPS (1  g/mL) and/or 1,25-Vit D3 (10−7 M) for 24 h. The inflammatory mediators (a) MIP-1β, (b) IL-8, (c) VEGF, and (d) IL-1β were measured in culture supernatants using cytometric bead array analysis. Each bar represents the mean ± SE ( –11). *Significantly different ( ) from LPS; **significantly different ( ) from control.
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(a) MIP-1β
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(b) IL-8
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(c) VEGF
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(d) IL-1β