Nuclear Factor-Kappa B Activity Regulates Brain Expression of P-Glycoprotein in the Kainic Acid-Induced Seizure Rats
Figure 2
PDTC and DEX pretreatment failed to prevent KA induced brain cell loss. (a) Representative photos of immunohistochemistry staining with anti-NeuN antibody (×100). Immunohistochemistry staining was performed to check the surviving neurons in the brain. The shape and number of neurons is normal in NS group (NS), while significant neuronal loss in the CA3 area was observed 24 h after KA micro-injection in the EP group (EP). In the KA induced rat pre-treated with PDTC (PDTC) or DEX (DEX), the survival neuron numbers did not significantly increase compared to the EP group. Additionally, the neuron number in dentate gyrus was not significantly changed among the four groups. Histogram showed the number of surviving neurons in the indicated brain area of each group (/HF). * versus NS group; ** versus NS group; # versus EP group, CA3, hippocampus CA3 area; DG: dentate gyrus; AMYG: amygdaloid nuclear. (b) Representative photos of TUNEL staining (times 200). Cells undergoing apoptosis were detected (labeled by FITC and shown in green) in each group 24 h after KA or vehicle injection. KA injection did not increase TUNEL positive cells significantly; there was no difference in TUNEL positive cell numbers between PDTC/DEX and EP groups. CA3, ; DG, ; AMYG, , by ONEWAY-ANOVA test; CA3, hippocampus CA3 area; DG: dentate gyrus; AMYG: amygdaloid nuclear.
