Mediators of Inflammation / 2011 / Article / Fig 5

Research Article

Novel Biphasic Role of LipoxinA4 on Expression of Cyclooxygenase-2 in Lipopolysaccharide-Stimulated Lung Fibroblasts

Figure 5

LipoxinA4 receptor is expressed in rat lung fibroblasts and upregulated by LPS. Rat lung fibroblast cells were treated with LPS (1 μg/mL) in the presence or absence of LXA4 (100 nM) of for 24 hours. mRNA was isolated and semiquantiative RT-PCR for LXA4R and β-actin were performed. Amplified cDNA was separated by 1.6% agarose gel electrophoresis, visualized with ethidium bromidem and analyzed by gel densitometry relative to β-actin expression. Lane 1: untreated control. Lane 2: LPS only (1 μg/mL). Lane 3: LPS (1 μg/mL) and LXA4 (100 nM). * versus control group, # versus (100 nM) LXA4 group. These data represent 6 individual experimental repeats.