NF-B p65 protein expression in the cerebellum of autistic subjects. (a) Two independent western blot studies on cerebellar homogenates using NF-B p65 antibody (dilution 1 : 500). Lanes 1–4 and 9–11 represent the controls, and lanes 5–8 and 12–14 represent the autistic subjects. The lower panel shows actin bands (MW: 48 kd), and the upper panel shows the NF-B p65 protein bands (MW: 65 kd). (b) The blots shown in (a) were quantitated after being normalized by actin. Data are shown as mean ± SE. White bars represent controls, and black bars represent autistic subjects. (c) Immunostaining of cerebellar sections using NF-B p65 antibody (dilution 1 : 150). Very faint expression of NF-B p65 (red color) was detected in the neural cells (indicated by arrows) in autistic cerebellar (right panel), as well as in the matched controls (left panel). The image is viewed under high power microscopy.
