Emerging Role of PPAR-β/δ in Inflammatory Process Associated to Experimental Periodontitis

Figure 4

Effect of GW0742 on iNOS expression. Positive staining for iNOS (a) and nitrotyrosine (c) was observed in gingivomucosal tissue after ligature. In gingivomucosal tissue of GW0742-treated rats, no positive staining was observed for iNOS (b) and nitrotyrosine (d). Densitometry analysis of immunocytochemistry photographs (𝑛=5 photos from each sample collected from all mice in each experimental group) for iNOS and nitrotyrosine was assessed (e). Moreover a significant increase in iNOS expression, assayed by western blot analysis, was detected in the tissue from ligature-treated rats (f, g). The treatment with GW0742 significantly reduced iNOS expression (f, g). This figure is representative of at least 3 experiments performed on different experimental days. A representative blot of lysates obtained from 5 animals per group is shown, and densitometry analysis of all animals is reported. The results in (g) are expressed as mean ± S.E.M. from 𝑛=5/6 gingivomucosal tissues for each group. *𝑃<0.01 versus nonligated group. °𝑃<0.01 versus ligated group.