A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells
The activity of microglial BV-2 cells induced by fluoride. Cells were treated with indicated concentrations of NaF for 24 h and immunocytochemistry localization with an OX-42 antibody as a microglial marker were observed. Microglial activity was detected by OX-42 expression, and the microglia cells treated with LPS were used as a positive control. Morphological changes of microglia from the resting state ((a) small cell bodies and thin, long, or ramified processes) to the activated state ((c), (d), (e), (f) larger cell bodies with short, thick) were observed after fluoride or LPS treatment in the BV-2 cells. High expression areas of OX-42 immunoreactivity were indicated by arrows. Optic microscopy: HE (400×). (a) control, (b) 1 mg/L NaF, (c) 5 mg/L NaF, (d) 10 mg/L NaF, (e) 20 mg/L NaF, and (f) 100 ng/mL LPS.